Action Members are any researcher who participates actively in PROTEOSTASIS. All Members belong to one or more Working Group. Members can include researchers from COST Countries, Near Neighbour and International Partner Countries.
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Sascha Martens Please log-in to see email and phone. |
University of Vienna. Max F Perutz Laboratories http://www.mfpl.ac.at Austria |
WG2 |
| Speciality: Biochemical and cell biological investigation of autophagy in eukaryotic cells. Skills: We specialize on biochemical reconstitution experiments using recombinantly expressed and purified proteins as well as liposomes. In addition, we study autophagy in the yeast S. cerevisiae and in mammalian cell lines. | |||
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Sebastien Leon Please log-in to see email and phone. |
Institut Jacques Monod - CNRS - Université Paris Diderot http://tinyurl.com France |
WG1 |
| Our team focuses on the regulation of membrane protein trafficking by ubiquitin, using the yeast Saccharomyces cerevisiae as a model system. We are particularly interested in understanding the molecular mechanisms governing plasma membrane proteome remodeling in the face of nutritional challenges (Becuwe et al., J Cell Biol 2012 ; Becuwe & Leon, eLife 2014). The key goals are to elucidate the mechanisms in charge of the nutrient-regulated ubiquitylation of transporters, and how this regulates their localization. Our approaches range from cell biology techniques (live cell microscopy, microfluidics, BiFC), genetics and biochemistry (in vivo and in vitro ubiquitylation, interaction proteomics) to systems-wide approaches (genetic screens, high-throughput imaging). | |||
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Selcuk Sozer Tokdemir Please log-in to see email and phone. |
ISTANBUL UNIVERSITY. RESEARCH INSTITUTE OF EXPERIMENTAL MEDICINE http://deneyseltip.istanbul.edu.tr Turkey |
WG5 |
| Speciality: Kanser stem cell, myeloproiferative neoplasm. Skills: I. Cell culture; 1. Stem cell and Cancer Stem Cell i. Hematopoietik stem cell ? Cord blood ? Bone Marrow ? Peripheral Blood ii. Endothelial Progenitor Cells ? Cord blood, Bone Marrow, Peripheral Blood, Solid Organs II. In vitro drug treatment III. Analysis of Differantiation, apoptosis, proliferation and cell cycle tests | |||
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Shenhav Cohen Please log-in to see email and phone. |
No website Israel |
WG1 |
| Speciality:We are interested in the molecular mechanisms of muscle atrophy and the specific role that the ubiquitin proteasome system plays in this process. We focus on the mechanisms promoting myofibril disassembly and degradation by probing the functions of certain atrophy-related ubiquitin ligases. Our goal is to identify key players in order to develop countermeasures to block or attenuate this debilitating process. Skills: We use a sophisticated in vivo gene transfection approach to study gene effects in normal or atrophying muscle of adult wild type mouse, in order to avoid developmental defects often seen in transgenic or knockout animals. Our work involves in vitro biochemical assays and in vivo experiments. | |||
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Silke Meiners Please log-in to see email and phone. |
Comprehensive Pneumology Center. Helmholtz Zentrum München http://www.cpc-munich.org Germany |
WG2 |
| The research in the CPC is focused on chronic lung diseases. We integrate state-of-the-art methods of molecular and cell biology, pharmacology, molecular pathology and clinical medicine in order to develop new diagnostical tools and therapies for chronic lung diseases. We have access to human lung tissue as well as to BAL and blood samples. Our small animal facility allows mouse experiments to identify novel pathomechanisms and to validate therapeutic targets for pulmonary fibrosis and smoke related lung diseases. Orientation of work: 1) Role of proteasome function in pathogenesis of chronic lung disease 2) Understanding and dissecting the role of proteasome regulators for proteostasis in cellular growth and differentiation 3) Development of smart nanoparticles for delivery of proteasome inhibitors | |||
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Simona Polo Please log-in to see email and phone. |
Fondazione IFOM Istituto FIRC di Oncologia Molecolare http://www.ifom-ieo-campus.it Italy |
WG3 |
| My major contributions have been on the understanding of how Ub works in the endocytic pathway, with a specific focus at the critical interface between endocytosis/trafficking and intracellular signaling. The results that I have obtained over the past years have contributed to establish new fundamental paradigms: 1) Ubiquitin is an endocytic signal in mammalian cells: 2) Ubiquitin is a signaling molecule 3) Ubiquitin ligase E3s, are regulated by ubiquitination | |||
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Solange Desagher Please log-in to see email and phone. |
Institute of Molecular Genetics of Montpellier. UMR5535 CNRS-University of Montpellier http://www.igmm.cnrs.fr France |
WG2 |
| The main goal of the group is to identify new molecular mechanisms controlling apoptosis in connection to the ubiquitin-proteasome system. We have identified a novel E3 ubiquitin-ligase, Trim17, that plays a crucial role in the triggering of apoptosis. Our results indicate that Trim17 is induced during neuronal apoptosis both in vitro and in vivo, that its expression is both necessary and sufficient for inducing neuronal death, and that its pro-apoptotic effect depends on its E3 activity. Moreover, we have demonstrated that Trim17 is an E3 ubiquitin-ligase for Mcl-1, an anti-apoptotic protein of the Bcl-2 family. Our current objectives are to elucidate the mechanisms of regulation of Trim17, to characterize its substrates and to determine its role in different models of both physiological and pathological apoptosis. We are notably studying the functional interaction between Trim17 and other proteins of the TRIM family, that constitutes a sub-group of RING-containing E3 ubiquitin-ligases. We are also studying the effect of Trim17 on the level and the activity of two different transcription factors. Interestingly, the interaction with Trim17 appeared to depend on the prior SUMOylation of one of these transcription factors. | |||
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Sonja Gisela Lorenz Please log-in to see email and phone. |
Rudolf Virchow Center for Experimental Biomedicine, University of Wuerzburg http://virchow.uni-wuerzburg.de Germany |
WG1 |
| Posttranslational modifications, ubiquitin, ubiquitination enzymes, enzyme mechanism, tyrosine kinases, protein-protein interactions, structural biology | |||
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Stamatis Rigas Please log-in to see email and phone. |
Agricultural University of Athens http://www.aua.gr Greece |
WG2 |
| Plant Developmental Biology | |||
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Stefan Hoth Please log-in to see email and phone. |
Universität Hamburg http://www.biologie.uni-hamburg.de Germany |
WG2 |
| We have a strong focus on plasma membrane-localized ubiquitin ligases that regulate development and stress responses in plants. We investigate these proteins on the molecular level (beginning with the resolution of their structure in collaboration with DESY) and on the functional level in planta. The major function studied is in the control of cell death and senescence. | |||
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Stefan Marciniak Please log-in to see email and phone. |
University of Cambridge http://www.cimr.cam.ac.uk UK |
WG3 |
| We wish to understand the cellular consequences of endoplasmic reticulum stress, in particular its effects on tissue growth and cell survival. In doing so, we hope to identify targets for the development of novel therapies. During endoplasmic reticulum stress, protein biosynthesis is initially attenuated through phosphorylation of the translation initiation factor eIF2? by the kinase PERK. Subsequent dephosphorylation of eIF2? following the induction of the phosphatase PPP1R15A (GADD34) restores protein translation. We previously discovered that this recovery of translation can contribute to the toxic effects of endoplasmic reticulum stress. This raises the exciting possibility that modulation of eIF2? phosphorylation may provide a useful target for the development of novel drugs to protect tissues from cell death. Cellular stresses frequently impair cell cycle progression, which can prejudice tissue growth. Using mammalian cell biology and Drosophila genetics we recently described a novel G2 cell cycle checkpoint initiated by translation attenuation during endoplasmic reticulum stress. This too provides potential targets for the development of new therapies. | |||
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Stefano Gastaldello Please log-in to see email and phone. |
Karolinska Institutet http://ki.se Sweeden |
WG1 |
| Muscle and SUMO. SUMO is a small ubiquitin-like protein and is reversible attached to a specific lysine in a multitude of proteins. Modification by SUMO induced by internal or external | |||
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[email protected] Please log-in to see email and phone. |
CEDOC- Centro de Estudos de Doenças Crónicas http://cedoc.unl.pt Portugal |
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| Molecular mechanisms of neurodegeneration in Parkinson's disease, using yeast models. Molecular mechanisms of retinal neurodegeneration in diabetes and Parkinson's disease. | |||
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Steven Tregay Please log-in to see email and phone. |
FORMA Therapeutics http://www.formatherapeutics.com USA |
WG1 |
| Deubiquitination, Sumoylation, E-ligases, and Epigenetic readers/writers / erasers and super-enhancers. | |||
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Stjepan Uldrijan Please log-in to see email and phone. |
Masaryk University, Faculty of Medicine http://www.med.muni.cz Czech Republic |
WG1 |
| Cell cultures, including induced pluripotent stem cells. Analyses of cellular proliferation and viability. Flow cytometry. Analyses of cellular energy metabolism. Molecular cloning. DNA and RNA transfections. Gene targeting using CRISPR/Cas9 system. Protein interaction and degradation assays. Ubiquitylation assays in cells. Fluorescence microscopy. Transmission electron microscopy. | |||
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Sulev Kõks Please log-in to see email and phone. |
University of Tartu. Department of Pathophysiology http://www.biomeditsiin.ut.ee Estonia |
WG6 |
| Genomics, RNAseq, animal experiments, the function of Wfs1 protein, ER stress in the inflammatory and degeneratiive diseases. We have studied psoriasis in the relation of ER stress and now work on the role of ER stress in the Parkinson disease. Available technologies are: Next generation sequencing, bioinformatics and statistics of NGS results, RNAseq, Exome seq, clinical biobank, animal center with in vivo imaging, transgenic facility. | |||
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Susana Seixas Please log-in to see email and phone. |
Genetic Diversity Unit. IPATIMUP http://www.ipatimup.pt Portugal |
WG3 |
| Analysis of the genetic variation of proteolysis genes (SERPINs, WFDCs and KLKs) in both healthy and disease individuals. Experimental assessment of gene variants impact in gene expression and regulation, protein folding and molecular pathways. Comparative genomics and evolutionary studies. | |||
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Sykiotis Gerasimos Please log-in to see email and phone. |
University Hospital of Lausanne (CHUV) http://www.chuv.ch Switzerland |
WG6 |
| My research focuses on the Nrf2 antioxidant response system and its protective roles in different systems and organisms, including flies, mice and humans. Nrf2 is a main convergence point of proteolytic and cell defense systems: it is not only activated by oxidative stress but it also cross-talks with the UPR through its activation by the endoplasmatic reticulum stress-responsive PERK kinase, and with the ALS through the interaction of sequestosome/p62 with the Nrf2 inhibitor Keap1. Moreover, Nrf2 controls the expression not only of antioxidant and detoxification genes but also of multiple proteasome subunits. Thus, Nrf2, which is itself degraded by proteolysis under normal conditions, coordinates the antioxidant response with the UPR and the ALS. My research will benefit from the networking opportunities within Proteostasis to launch new research projects on the relationship between Nrf2, UPR, and ALS signaling. As a practicing physician, I could also participate in clinically relevant projects launched by the Action. Conversely, Action participants will benefit from my expertise on Nrf2 signaling, including the experimental tools for its study at my disposal, which I can distribute freely.Skills: Clinical endocrinology and diabetology (adults, adolescents).Medical genetics and molecular genetic diagnostics.Molecular biology and model organism genetics (flies, mice).Drug discovery and clinical trials.Project evaluation (FP7, Marie Curie) | |||
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Sylvie Urbé Please log-in to see email and phone. |
Institute of Translational Medicine. University of Liverpool http://pcwww.liv.ac.uk United Kingdom |
WG3 |
| Endocytosis, RTK trafficking and signaling. Deubiquitylation in pathways germane to cancer. | |||
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Teresa Rinaldi Please log-in to see email and phone. |
UNIVERSITY OF ROME LA SAPIENZA DIP BIOLOGY and BIOTECHNOLOGY http://bbcd.bio.uniroma1.it Italy |
WG2 |
| Speciality: mitochondria and proteasome, CSN complex in yeast. Skills: yeast genetics and cellular biology, fluorescent and confocal microscopy. | |||
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Teresa Zoladek Please log-in to see email and phone. |
Institute of Biochemistry and Biophysics Polish Academy of Sciences (IBB PAS) http://www.ibb.waw.pl Poland |
WG2 |
| Molecular and cellular genetics and biology of yeast.Studies on the role of Rsp5-dependent ubiquitination of proteins in mechanisms of protein transport via endocytosis, autophagy, Golgi to ER transport.Role of Rsp5-dependent ubiquitination in actin cytoskeleton organization.Role of ubiquitination in tRNA metabolism. Regulation of Rsp5 ubiquitin ligase by phosphorylation. One goal of the newest grant is to find mechanisms which are disturbed in cells by mutations in VPS13A and VPS13B gene corresponding to human inherited diseases, Cohen syndrome and Chorea acanthocytosis, by using yeast as a model organism. Yeast Vps13 protein is involved in Golgi-to vacuole trafficking and was also found in actin cortical patches which are sites of endocytosis. Vps13 protein shows some homology to Atg2 protein involved in autophagy. Another goal is to find function of Atg2 in autophagy. | |||
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Theodora Farmaki Please log-in to see email and phone. |
CERTH-INAB http://inab.certh.gr Greece |
WG2 |
| 1. Study of the role of FKBP chaperones in seed germination under stress conditions (especially salinity). 2. Involvement of FKBP chaperones in autophagy, proteosome and SUMOylation. 3. FKBP interaction with PI3,5P2 and PI3P involved in autophagosome formation. 4. Role of phospholipases in cotton resistance to cold stress and wounding. | |||
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Thimo Kurz Please log-in to see email and phone. |
Scottish Institute for Cell Signaling. University of Dundee. SCILLS http://www.ppu.mrc.ac.uk United Kingdom |
WG1 |
| My lab focuses on various aspects of the ubiquitin/UBL system. We?re specifically interested in the UBL NEDD8 and how it regulates the largest class of Ubiquitin-ligases, the Cullin-RING E3s. We have also a focus on the role of the ubiquitin system in disease. In particular, we?ve recently focused on the role of a CUL3-based CRL in blood pressure regulation and on the involvement of the ubiquitin-receptor UBQLN2 in neurodegenerative diseases. | |||
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Thomas Sommer Please log-in to see email and phone. |
Max-Delbrück-Center for Molecular Medicine and Humboldt-University Berlin http://www.mdc-berlin.de Germany |
WG1 |
| Ubiquitin Proteasome Pathway, Protein Quality Control, ERAD, Membrane Protein Complexes | |||
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Thorsten Hoppe Please log-in to see email and phone. |
Institute for Genetics and CECAD Cluster of Excellence http://www.cecad.uni-koeln.de Germany |
WG4 |
| Speciality:The maintenance of protein homeostasis, or proteostasis, involves the degradation of misfolded and damaged proteins and is essential for cellular function, organismal growth, and ultimately viability. The integrity of the proteome is a long-term challenge not only for individual cells but also for entire organisms since damaged and aggregated proteins accumulate with stress and aging. The ubiquitin/proteasome system (UPS) and autophagy are the major proteolytic routes embedded in a cellular quality control network that ensure efficient turnover of defective proteins. Research in my lab aims to understand proteolytic networks -especially ubiquitin based - in aging and disease. Current projects address proteostasis mechanisms focused on genome stability, protein aggregation diseases and lifespan regulation. Skills: Use of Caenorhabditis elegans as a multicellular organism to study the physiological relevance of proteostasis mechanisms. Techniques include genetic approaches, forward genetic screens, reverse genetics, phenotypic analysis including life span, protein aggregation, muscle degeneration, neuronal function, in vivo degradation assays, biochemistry, time lapse microscopy, in vivo localization, next generation sequencing of genomic DNA, mRNAs, microRNAs. | |||
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Thorsten Pfirrmann Please log-in to see email and phone. |
Institute of Physiological Chemistry http://www.medizin.uni-halle.de Germany |
WG1 |
| We are mostly interested in UPS components involved in human genetic disease and in early development of Xenopus laevis. My focus lies on Ube3a, Otud3 and the CTLH complex. Additionally, we are studying the impact of protein glycation on proteostasis. | |||
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Tiago Fleming Outeiro Please log-in to see email and phone. |
University Medical Center Goettingen (UMG) http://www.med.uni-goettingen.de Germany |
WG1 |
| In our work we focus on the study of protein misfolding and aggregation and how this relates to neurodegenerative diseases such as Alzheimer?s, Parkinson?s, or Huntington?s disease. We use a variety of model organisms, from yeast to mouse models, and we employ cell and molecular biology techniques applied to neuroscience. We hold expertise in fluorescence microscopy (high-content imaging, FRAP, STED), molecular biology, lentiviral vector production, primary neuronal cultures, mouse models, protein purification, aggregation assays. | |||
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Tijana Stankovic Please log-in to see email and phone. |
Institute for Biological Research Sinisa Stankovic http://www.ibiss.bg.ac.rs Serbia |
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| RNA and DNA isolation | |||
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Tom Nicholson Please log-in to see email and phone. |
VIVA Bioscience Ltd. http://www.vivabioscience.com United Kingdom |
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Tommer Ravid Please log-in to see email and phone. |
The Hebrew University of Jerusalem http://www.bio.huji.ac.il Israel |
WG4 |
| Speciality: Protein quality control by the ubiquitin-proteasome system. Skills: We use yeast as a model organism, employing a large number of genetic and molecular biology tools. We also employ biochemical and cell biology assays for studying misfolded protein ubiquitylation and degradation, both in vitro and in vivo. We also take a high throughput approach for studying the biochemical properties of misfolded proteins. | |||